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Analysis of Monoliths for Chromatography of Gene Therapy Vectors and Other Proteins

Part of the BTEC Fall 2019 Seminar Series

Michael Ladisch, Ph.D.
Distinguished Professor of Agricultural & Biological Engineering, Purdue University

Seminar description

Monoclonal antibodies have molecular weights ranging from 140 to 150 kD, compared to adeno-associated viruses, made up of proteins (MW of 62 to 87 kD) that form capsids in the 100 nm range and function as gene therapy vectors. These size ranges present an opportunity for monolithic size exclusion chromatography columns consisting of single continuous structures, which form packed beds that are hydraulically stable at high linear velocities and chemically compatible with the purification of large proteins. Analysis of published experiments carried out with microscale systems and fibrous monoliths provides insights on how mass transfer effects in these types of stationary phases might be used to develop process-scale purification tools for high molecular weight molecules and gene therapy vectors.