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AAV Quality Attributes: Theory and Practice

Take this course to experience a 3-day, hands-on, overview of AAV Quality Attributes. Step through each CQA category, Identity, Quantity, Potency, Purity and Safety through interactive lectures, group workshops, and hands-on labs in BTEC’s state of the art facility. All course content is directly related to current applications in industry while providing basic theory to explain the underlying science.

Attendees will take a 3-day journey through the AAV Quality Attributes and CQAs. For each quality attribute, you will learn:

  • Specific characteristics to evaluate 
  • Commonly utilized and cutting-edge techniques
  • Regulatory expectations and industry trends 

Participants will take a deeper dive into some of the key analytical techniques with hands-on labs and workshops:

  • ddPCR lab and workshop
  • Transmission Electron Microscopy (TEM) lab
  • Capillary Gel Electrophoresis (CGE) lab and workshop
  • High-performance Liquid Chromatography: Size Exclusion column with Multi-Angle Light Scattering (HPLC-SE / MALS) lab and workshop
  • Analytical Ultracentrifugation (AUC) session
  • Next Generation Sequencing workshop
DatesCourseCostRegistration
May 12-13, 2026Artificial Intelligence in FDA-Regulated Manufacturing: From Foundations to Implementation$1,500Register
May 20–21, 2026Hands-On Essentials of Automation for Biomanufacturing$2,050Register
May 27–29, 2026Preparative Chromatography Column Packing: Lab to Process Scale$2,500Register
June 3–4, 2026Applied Cleaning Validation Practices: A STERIS Master Class$2,000Register
June 9–10, 2026Introduction to Biopharmaceutical Lyophilization$1,975Register
June 11, 2026Essentials of Spray Drying and Powder Analysis$995Register
June 16–18, 2026Fundamentals of Mammalian Cell Line Development$3,100Register
June 23–25, 2026Biopharmaceutical Assay Essentials$3,100Register
June 23–25, 2026Fermentation Engineering$3,100Register
June 30–July 2, 2026Hands-On Principles of mRNA Technology$2,500Register
July 8–10, 2026Downstream Biopharmaceutical Processes: Fundamentals and Design$3,100Register
July 14–16, 2026AAV Quality Attributes: Theory and Practice$2,975Register
July 14–17, 2026Hands-On cGMP Biomanufacturing Operations$4,200Register
July 28–31, 2026Hands-On Biomanufacturing of Vectors for Gene Therapy$4,500Register
Aug 6, 2026Design of Experiments for Biomanufacturing$500*Register
Sept 29–Oct 1, 2026Cell Culture Engineering$4,200Register
Oct 13–16, 2026Applied Principles and Techniques of Depth Flow Filtration (DFF) and Tangential Flow Filtration (TFF) for BioPharm Purification$3,995Register
Nov 10–12, 2026PAT for Real-time Culture Monitoring and Closed-Loop Process Control$2,975Register
November 12-19Microbial Contamination Control in Bioprocessing Operations$2,975

Register by June 29

Attend and you will learn 

  • What differentiates gene therapy viral vector Quality Attributes and why — history, basic biology, and manufacturing processes
  • Current industry and regulatory expectations, including future trends 
  • What is being characterized and/or measured for each Quality and Critical Quality Attribute category, how and why
  • The current landscape of analytical tests used in AAV development and manufacturing
  • How and why key AAV assays are performed with hands-on experience in BTEC’s biomanufacturing training labs: ddPCR
  • Special sessions on measuring Empty vs Full Capsids, Analytical Ultracentrifugation (AUC) and EMA EPARs (initial marketing authorization documents)

Delivery methods

50% Lecture
20% Discussion/group activity
30% Hands-on lab experience

Course content

30% Fundamentals and concepts
50% Industry applications
20% Emerging technologies
24 hours of instruction

Who should attend

  • The course will benefit those new to gene therapy or looking to expand their knowledge. Our list of satisfied participants includes those from biopharmaceutical companies, contract research (CROs), contract development and manufacturing organizations (CDMOs), pharmaceutical vendors, suppliers, and distributors, equipment sales, and trainers in the biopharmaceutical discipline. You will leave the course with a better understanding of the gene therapy regulatory landscape and its associated analytical methods that will enhance your career at any stage.
  • We welcome those who are:
  • New to the field or transitioning to tackle gene therapy
  • Individuals interested in cross-training
  • Managers and directors wishing to broaden their expertise
  • Quality assurance professionals interested in science and theory
  • Sales professionals looking to understand their products better

Custom offerings for groups of 4 or more are available on request. Contact BTEC for more information.

Professional certificate

This course can count toward your Special Topics Professional Certificate in Biomanufacturing Science.

Instructors 

Important Information for Course Participants

Please read about BTEC’s policies on payment, cancellation, pre-course communication and other important information for in-person courses offered on site at BTEC.