Skip to main content
NC State Home

Cell Culture Engineering

Learn key concepts and practical skills in bioprocess development and cell culture engineering in biomanufacturing. Lectures focus on cell culture media and feeding strategies, operational modes (batch, fed-batch, perfusion), bioreactor design (single-use and stainless steel), high-density cell banking, critical process controls, harvest operations, design of experiments (DOE) in process development, glycan analysis by mass spectrometry, and process analytical technology (PAT). Laboratory sessions provide hands-on experience in media preparation and filter integrity testing, single-use and stainless-steel bioreactor operation, rocker bag assembly inoculation and sampling, and high-density cell banking from perfusion cultures.

DatesCourseCostRegistration
May 12-13, 2026Artificial Intelligence in FDA-Regulated Manufacturing: From Foundations to Implementation$1,500Register
May 20–21, 2026Hands-On Essentials of Automation for Biomanufacturing$2,050Register
May 27–29, 2026Preparative Chromatography Column Packing: Lab to Process Scale$2,500Register
June 3–4, 2026Applied Cleaning Validation Practices: A STERIS Master Class$2,000Register
June 9–10, 2026Introduction to Biopharmaceutical Lyophilization$1,975Register
June 11, 2026Essentials of Spray Drying and Powder Analysis$995Register
June 16–18, 2026Fundamentals of Mammalian Cell Line Development$3,100Register
June 23–25, 2026Biopharmaceutical Assay Essentials$3,100Register
June 23–25, 2026Fermentation Engineering$3,100Register
June 30–July 2, 2026Hands-On Principles of mRNA Technology$2,500Register
July 8–10, 2026Downstream Biopharmaceutical Processes: Fundamentals and Design$3,100Register
July 14–16, 2026AAV Quality Attributes: Theory and Practice$2,975Register
July 14–17, 2026Hands-On cGMP Biomanufacturing Operations$4,200Register
July 28–31, 2026Hands-On Biomanufacturing of Vectors for Gene Therapy$4,500Register
Aug 6, 2026Design of Experiments for Biomanufacturing$500*Register
Sept 29–Oct 1, 2026Cell Culture Engineering$4,200Register
Oct 13–16, 2026Applied Principles and Techniques of Depth Flow Filtration (DFF) and Tangential Flow Filtration (TFF) for BioPharm Purification$3,995Register
Nov 10–12, 2026PAT for Real-time Culture Monitoring and Closed-Loop Process Control$2,975Register
November 12-19Microbial Contamination Control in Bioprocessing Operations$2,975

Register by September 14

Attend and you will learn 

  • Understand the principles of cell culture, media formulation and feeding strategies for optimal productivity.
  • Compare operational modes—batch, fed-batch, and perfusion—and identify their applications in biomanufacturing.
  • Evaluate single-use versus stainless-steel bioreactor designs for different process needs.
  • Identify and control critical process parameters in bioreactor operations.
  • Describe harvest operations and their role in maintaining product quality.
  • Utilize Design of Experiments (DOE) to optimize cell culture processes.
  • Interpret glycan analysis data generated by mass spectrometry.
  • Understand the role of Process Analytical Technology (PAT) in real-time process monitoring.
  • Perform hands-on bioprocess tasks, including media preparation, filter integrity testing, rocker bag assembly, bioreactor inoculation, sampling, and high-density cell banking.

Delivery methods

45% Lecture
5% Discussion/group activity
50% Hands-on lab experience

Course content

50% Fundamentals and concepts
30% Industry applications
20% Emerging technologies
22.5 hours of instruction

Who should attend

  • Professionals with some biopharmaceutical experience
  • Professionals within the biotech industry who want to gain an understanding of upstream processing
  • Professionals involved in bioreactor equipment design and biopharmaceutical facilities design

Professional certificate

This course can count toward your Upstream Professional Certificate in Biomanufacturing Science.

Lead Instructors 

Important Information for Course Participants

Please read about BTEC’s policies on payment, cancellation, pre-course communication and other important information for in-person courses offered on site at BTEC.