Applied Principles and Techniques of Depth Flow Filtration (DFF) and Tangential Flow Filtration (TFF) for BioPharm Purification
By participating in this comprehensive four-day filtration course, you will gain a solid understanding of the core principles and best practices for both depth filtration (DFF) and tangential flow filtration (TFF) as applied in modern biopharmaceutical upstream and downstream purification processes. This program is designed to equip you with practical skills and insights that can be directly implemented in your work environment in the areas of Process Development, Manufacturing, and Quality Assurance/Validation.
| Dates | Course | Cost | Registration |
|---|---|---|---|
| May 12-13, 2026 | Artificial Intelligence in FDA-Regulated Manufacturing: From Foundations to Implementation | $1,500 | Register |
| May 20–21, 2026 | Hands-On Essentials of Automation for Biomanufacturing | $2,050 | Register |
| May 27–29, 2026 | Preparative Chromatography Column Packing: Lab to Process Scale | $2,500 | Register |
| June 3–4, 2026 | Applied Cleaning Validation Practices: A STERIS Master Class | $2,000 | Register |
| June 9–10, 2026 | Introduction to Biopharmaceutical Lyophilization | $1,975 | Register |
| June 11, 2026 | Essentials of Spray Drying and Powder Analysis | $995 | Register |
| June 16–18, 2026 | Fundamentals of Mammalian Cell Line Development | $3,100 | Register |
| June 23–25, 2026 | Biopharmaceutical Assay Essentials | $3,100 | Register |
| June 23–25, 2026 | Fermentation Engineering | $3,100 | Register |
| June 30–July 2, 2026 | Hands-On Principles of mRNA Technology | $2,500 | Register |
| July 8–10, 2026 | Downstream Biopharmaceutical Processes: Fundamentals and Design | $3,100 | Register |
| July 14–16, 2026 | AAV Quality Attributes: Theory and Practice | $2,975 | Register |
| July 14–17, 2026 | Hands-On cGMP Biomanufacturing Operations | $4,200 | Register |
| July 28–31, 2026 | Hands-On Biomanufacturing of Vectors for Gene Therapy | $4,500 | Register |
| Aug 6, 2026 | Design of Experiments for Biomanufacturing | $500* | Register |
| Sept 29–Oct 1, 2026 | Cell Culture Engineering | $4,200 | Register |
| Oct 13–16, 2026 | Applied Principles and Techniques of Depth Flow Filtration (DFF) and Tangential Flow Filtration (TFF) for BioPharm Purification | $3,995 | Register |
| Nov 10–12, 2026 | PAT for Real-time Culture Monitoring and Closed-Loop Process Control | $2,975 | Register |
| November 12-19 | Microbial Contamination Control in Bioprocessing Operations | $2,975 |
Register by September 28
Attend and you will learn
The comprehensive lecture sections is expertly crafted to equip participants with a thorough understanding of the key parameters and factors influencing DFF and TFF membrane performance, as well as product recovery, for both upstream cell clarification and downstream UF/DF biopharmaceutical applications. The depth filtration (DFF) section explores the latest in polymeric membrane and matrix media technologies, while the tangential flow filtration (TFF) portion delves into both flat sheet cassette and hollow fiber element configurations, highlighting innovative advancements in the field.
Spanning the four immersive days, the hands-on laboratory workshop enables participants to conduct DFF and TFF application trials using cutting-edge benchtop systems. The curriculum dedicates two full days to cell clarification—first with DFF and then with TFF—followed by an in-depth focus on UF/DF by TFF on the third day. Data generated during these practical sessions will be thoroughly summarized, discussed, and applied to real-world scale-up challenges and SOP development. All workshop activities are grounded in the fundamental principles and techniques presented in the prerequisite lecture, ensuring a seamless integration of theory and practice.
Delivery methods
25% Lecture
10% Discussion/group activity
65% Hands-on lab experience
Course content
50% Fundamentals and concepts
30% Industry applications
20% Emerging technologies
29.75 hours of instruction
What makes this filtration lecture and workshop different
- Technology Focus and Not Sales/Marketing Focus — Unbiased and comprehensive lecture and workshop presenting the full spectrum of the acceptable technologies that no single vendor can offer. Both tubular (hollow fiber) and flat sheet membrane (cassette) formats are discussed and used in the workshop to provide comparable performance analysis.
- Application Expertise — Depth and content of information presented and covered is unprecedented to other presentations. An interactive lecture that focuses on current issues and problems. The lecturer makes himself available to address each trainee’s current issues.
- Applied Principles — Proven methods that lead to successful results and data interpretations from an instructor with over 40-years of application hands-on experience.
- Diagnostic Tool — Membrane results are predictable—their performance is directly consistent to the principles and with any deviation in membrane performance—thus, analysis can be used to identify the process condition or design contributing to the cause.
- Summary — How to design the appropriate setup, how to run the required procedures properly, what data to record, how to summarize the data, and how to interpret the data.
Who should attend
Engineers, scientists, managers, technical personnel, and researchers involved in biopharmaceutical filtration processes, with varying levels of knowledge.
Instructor
John Rozembersky is the founder and president of Rozembersky Group Inc (RGI), an independent biofiltration consulting firm founded in 2005.
With over 45 years of hands-on experience and in-depth knowledge in membrane and membrane bio-application technologies, and process systems, John is internationally recognized as a leading authority in Tangential Flow Filtration (TFF) in the biopharmaceutical sector from small scale bench-top development to large scale manufacturing process for both flat sheet membrane cassettes and hollow fiber membrane elements.
In the past decade, John has played a major lead role with teams that design, manufacture, implement and provide hands-on support and training to a full range of ultimate single-use TFF systems from bench scale to large scale manufacturing with solutions that resolve current major process and application pain points.