Learn key concepts and practical skills in bioprocess development and cell culture engineering in biomanufacturing. Lectures focus on cell culture media and feeding strategies, operational modes (batch, fed-batch, perfusion), bioreactor design (single-use and stainless steel), high-density cell banking, critical process controls, harvest operations, design of experiments (DOE) in process development, glycan analysis by mass spectrometry, and process analytical technology (PAT). Laboratory sessions provide hands-on experience in media preparation and filter integrity testing, single-use and stainless-steel bioreactor operation, rocker bag assembly inoculation and sampling, and high-density cell banking from perfusion cultures.